Functional Organization of the Nucleus: A Laboratory Guide
Barbara A. Hamkalo and Sarah C.R. Elgin (Eds.)
A text intended for researchers and students in cell biology, genetics and molecular biology, developmental biology, biochemistry and biophysics. The complex problem of the relationship between nuclear structure and function demands a multidisciplinary, multifaceted approach. This laboratory guide is designed for researchers, from graduate students to professors, who need detailed protocols and general discussions on a broad range of techniques. The volume presents a selection of different methodological approaches for the analysis of nuclear structure and function - from cytological to molecular to genetic. These include visualization of nucleic acid sequences using hybridization probes, visualization of proteins using immunological probes, isolation of chromatin fractions, mapping "in vitro" protein-DNA interactions, reconstitution of functional templates and nuclear substructures, and genetic approaches to identifying and characterizing chromosomal components.
年:
1991
出版社:
Academic Press
语言:
english
页:
691
ISBN 13:
9780125641357
系列:
Methods in Cell Biology 35
文件:
PDF, 33.70 MB
IPFS:
,
english, 1991